Eric Greene is a Professor in the Department of Biochemistry & Molecular Biophysics at Columbia University. Dr. Greene’s laboratory pioneered development of novel technologies for studying protein–nucleic acid interactions at the single–molecule level using real–time optical microscopy with an overarching goal of seeking to understand the molecular mechanisms that cells use to repair, maintain, and decode their genetic information. This research combines aspects of biochemistry, physics, and nanoscale technology to address complex biological problems that cannot easily be addressed through traditional biochemical or genetic approaches. The advantages of the technologies developed in the Greene group are that enable one to see what proteins are bound to DNA, where they are bound, how they move, and how they interact with and influence partner proteins – all at the level of biochemical single reactions. Using these approaches, the Greene laboratory has tackled problems related to: understanding how CRIPSR systems locate specific DNA target site sites; understanding how the SMC (structural maintenance of chromosomes)complex condensin contributes to higher–order chromosome organization; and defining the mechanisms involved in homologous DNA recombination.